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Title: Ion-exchange chromatography (Experiment F) Objective: To find out the concentration of NH4OH at which tyrosine are eluted from the cation exchanger by using gradient elution technique. Introduction: Ion-exchange chromatography is the technique used to separate protein molecules according to their molecular net charge via column materials that carry an opposite charge. There are two types of ion exchangers, cation exchangers and anion exchangers. Cation exchangers are those with chemically bound negative charges, which counterbalance by cations. Anion exchangers are those with chemically bound positive charges which